Team:Warsaw/Calendar-Main/23 June 2008

From 2008.igem.org

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<h4>Michał K.</h4>
<h4>Michał K.</h4>
<p>
<p>
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Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin).</p>
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Inoculation other separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha </a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p>
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<h3>Preparation of alpa-A and omega-A fusions</h3>
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<h3>Preparation of alpha-A and omega-A fusions</h3>
<h4>Michał L., Ewa, Marcin</h4>
<h4>Michał L., Ewa, Marcin</h4>
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<p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other two. PCRs were repeated. New annealing temp.: 48&deg;C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p>
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<p>We have successfully amplified Omega-linker (<a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/23_June_2008#fig1">Fig. 1</a>), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48&deg;C. Mg<sup>2+</sup> concentration elevated to 4 mM.</p>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a>
<a name="fig1"><img src="https://static.igem.org/mediawiki/2008/3/36/PCR_Alinker_Omegalinker_WAW.jpg"width=200/></a>
<var><b>Fig. 1.</b>PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.</var>
<var><b>Fig. 1.</b>PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.</var>

Revision as of 21:38, 12 October 2008

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Preparation of constructs with OmpA protein fusions

Michał K.

Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin).

Preparation of alpha-A and omega-A fusions

Michał L., Ewa, Marcin

We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM.

Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.

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