Team:Warsaw/Calendar-Main/21 August 2008

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<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4>
<h3>Cloning of protein A DNA to GeneArt plasmid</h3><h4>Antoni</h4>
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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pGeneart+A Primers used:
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<li> Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on colonies from plates with transformations pGeneart+A. <br>Primers used:
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AL+SacI">AL+SacI</a>. </li>

Revision as of 21:54, 12 October 2008

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Cloning of protein A DNA to GeneArt plasmid

Antoni

  1. Colony PCR on colonies from plates with transformations pGeneart+A.
    Primers used: AP+NotI and AL+SacI.
  2. Confirmed transformant colonies inoculated to liquid LB with ampicillin.
  3. Inoculation to liquid LB with ampicillin: pET15b+OmpA-alpha.

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