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Team:Warsaw/Calendar-Main/27 August 2008
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| - | <h3>Cloning of protein Z DNA to pET15b+Omp- | + | <h3>Cloning of protein Z DNA to pET15b+Omp-alpha plasmid</h3><h4>Antoni</h4> |
<p> Transformant colonies inoculated to liquid LB with ampicillin.</p> | <p> Transformant colonies inoculated to liquid LB with ampicillin.</p> | ||
| - | <h3>Cloning of protein A DNA to pET15b+Omp- | + | <h3>Cloning of protein A DNA to pET15b+Omp-alpha plasmid</h3><h4>Michał K.</h4> |
| - | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pGeneart+A and pET15b+Omp- | + | <ol><li> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">Digest</a> of pGeneart+A and pET15b+Omp-alpha with NdeI and NotI (Orange buffer), pET15b was also <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#removing"> dephosphorylated</a>. </li> |
<li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> | <li> Gel electrophoresis of digested plasmids and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper bands (400 bp for A lane and 6200 bp for pET15b lane). </li> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+ | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pET15b+alpha and A. </li> |
<li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>. </li> | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>. </li> | ||
<li> Transformants plating on LB + ampicillin.</li> | <li> Transformants plating on LB + ampicillin.</li> | ||
Revision as of 22:01, 12 October 2008
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Cloning of protein Z DNA to pET15b+Omp-alpha plasmidAntoniTransformant colonies inoculated to liquid LB with ampicillin. Cloning of protein A DNA to pET15b+Omp-alpha plasmidMichał K.
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