Team:Warsaw/Calendar-Main/4 July 2008

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<h4>Paweł</h4>
<h4>Paweł</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega). </li>  
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from bacterial cultures inoculated on previous day (<a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA_alpha</a> and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-omega>pET15b+OmpA_omega</a>). </li>  
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with SacI and BamHI (BamHI buffer). </li>
<li> Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest">digest</a> of plasmids with SacI and BamHI (BamHI buffer). </li>
<li> Gel electrophoresis. </li>
<li> Gel electrophoresis. </li>

Revision as of 22:57, 12 October 2008

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Preparation of constructs with OmpA protein fusions

Paweł

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega).
  2. Control digest of plasmids with SacI and BamHI (BamHI buffer).
  3. Gel electrophoresis.



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