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Team:Warsaw/Calendar-Main/10 July 2008
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<li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and NotI (BamHI buffer). | <li> Control <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#digest">digest</a> of isolated plasmids with BamHI and NotI (BamHI buffer). | ||
</li> | </li> | ||
| - | <li> Gel electrophoresis - we confirmed pCACYC177 + OmpA_omega. We didn't obtain pCACYC177 + OmpA_alpha probably because of a mistake in plating. </li> | + | <li> Gel electrophoresis - we confirmed <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pCACYC177 + OmpA_omega</a>. We didn't obtain <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pCACYC177 + OmpA_alpha</a> probably because of a mistake in plating. </li> |
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of pACYC177 and OmpA_alpha (1 hr).</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177>pACYC177</a> and OmpA_alpha (1 hr).</li> |
| - | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products: pACYC177 and OmpA_alpha.</li> | + | <li> Transformation of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with ligation products: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177>pACYC177</a> and OmpA_alpha.</li> |
<li> Transformants plating on LB + kanamycin.</li> | <li> Transformants plating on LB + kanamycin.</li> | ||
</ol></p> | </ol></p> | ||
<html> | <html> | ||
| - | <h3>Preparation of construct pKS with A protein</h3> | + | <h3>Preparation of construct <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> with A protein</h3> |
<h4>Michał L., Marcin</h4> | <h4>Michał L., Marcin</h4> | ||
<p><ol> | <p><ol> | ||
<li> Inactivation of digestion enzymes and CIAP.</li> | <li> Inactivation of digestion enzymes and CIAP.</li> | ||
| - | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested PCR product and pKS 2h at room temperature.</li> | + | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested PCR product and <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a> for 2h at room temperature.</li> |
<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with 7 µl of ligation mix.</li> | <li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Transformation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a> strain with 7 µl of ligation mix.</li> | ||
<li> Transformants plating on LB + ampicillin + X-gal + IPTG.</li></ol></p> | <li> Transformants plating on LB + ampicillin + X-gal + IPTG.</li></ol></p> | ||
Revision as of 23:09, 12 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.
Preparation of construct pKS with A proteinMichał L., Marcin
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