Team:Warsaw/Calendar-Main/25 September 2008

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<p>Treatment of mutageneses as on <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008>23rd September</a>. </p>
<p>Treatment of mutageneses as on <a href=https://2008.igem.org/Team:Warsaw/Calendar-Main/23_September_2008>23rd September</a>. </p>
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<h3>Preparation of alpha_A construct</h3>
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<h4>Antoni</h4>
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<li> <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on alpha_linker and linker_A with <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AlphaL+SacI">AlphaL+SacI</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#AP+NotI">AP+NotI</a> primers and 10% DMSO (30 cycles, elongation 40&nbsp;s, annealing temperature 72&deg;C). </li>
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<li> Gel electrophoresis of PCR products and <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#DNA_isolation_from_agarose_gel">gel-out</a> of proper band (1019 bp).</li>
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<li>Measurment of concentration of both isolated products.</li>
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</ol></p>
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Revision as of 23:06, 13 October 2008

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Mutagenesis of protein A

Paweł

Treatment of mutageneses as on 23rd September.

Preparation of alpha_A construct

Antoni

  1. PCR on alpha_linker and linker_A with AlphaL+SacI and AP+NotI primers and 10% DMSO (30 cycles, elongation 40 s, annealing temperature 72°C).
  2. Gel electrophoresis of PCR products and gel-out of proper band (1019 bp).
  3. Measurment of concentration of both isolated products.

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