Team:Warsaw/Calendar-Main/25 August 2008

From 2008.igem.org

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<h3>Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega</h3><h4>Michał K.</h4>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#ligation">Ligation</a> of digested vectors from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008">14 August</a> and alpha DNA fragment from <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/14_August_2008">14 August</a> (more vectors added).</li>
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<li>  <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#electrotransform">Electroporation</a> of <i>E. coli</i> <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">TOP10</a>  with ligations products.</li>
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<li>  Transformants plating on LB + kanamycine.</li></ol>

Revision as of 23:12, 13 October 2008

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Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Antoni

  1. Transformation of E. coli TOP10 strain with friday's ligation products.
  2. Transformants plating on LB + ampicillin.

Cloning of alpha to pACYC177+OmpA_A_omega and pACYC177+OmpA_Z_omega

Michał K.

  1. Ligation of digested vectors from 14 August and alpha DNA fragment from 14 August (more vectors added).
  2. Electroporation of E. coli TOP10 with ligations products.
  3. Transformants plating on LB + kanamycine.

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