Team:Imperial College/BioBricks

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Revision as of 16:40, 15 October 2008

BioBricks & Characterisation

Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.

Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.

Parts Submitted to the Registry
For now, here are the parts we will submit to the Registry. They can also be accessed here.

Production Status
This table shows our progress with each part:

Key
Submitted
Sequenced
Midiprepped
Fully verified
Partially Verified
Transformed
Ligation pending/Failed
Awaiting GeneArt
Cloning difficulties

Part Number	Part Name	Status
K143001	5' AmyE Integration Sequence	Sequenced
K143002	3' AmyE Integration Sequence	Sequenced
K143005	5' EpsE Integration Sequence	PCR Verified
K143006	3' EpsE Integration Sequence	Sent for Sequencing
K143008	5' PyrD Integration Sequence	Pending
K143009	3' PyrD Integration Sequence	PCR verified
K143031	Aad9 Spectinomycin Resistance Gene	Sent for Sequencing
K143032	EpsE Molecular Clutch Gene of B. subtilis	Sequenced
K143033	LacI (Lva^-, N terminal deletion)	Sequenced
K143034	LipA-EAK16 Fusion	Partially Sequenced
K143035	LipA-Human Elastin Fusion	Transformed
K143036	Xylose Operon Regulatory Protein	PCR verified
K143037	Ytva Blue Light Receptor of B. subtilis	PCR verified
K143038	SacB-EAK16 Fusion	Sequenced
K143039	SacB-Human Elastin Fusion	Fails to clone
K143050	P43-gsiB	Sent for Sequencing
K143051	P43-spoVG	Sequenced
K143052	Pveg-gsiB	Sequenced
K143053	Pveg-spoVG	Sequenced
K143054	Phyperspank-gsiB	Pending
K143055	Phyperspank-spoVG	PCR verified
K143056	Pxyl-gsiB	Pending
K143057	Pxyl-spoVG	Pending
K143058	Pctc-gsiB	Midiprepped
K143059	Pctc-spoVG	Midiprepped
K143060	PgsiB-gsiB	Wrong insert cloned
K143061	PgsiB-spoVG	Sequenced
K143062	LacI - Terminator	Pending
K143063	XylR - Terminator	Pending
K143064	CAT - Terminator	Sent for Sequencing
K143065	Aad9 - Terminator	Pending

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 This table shows our progress with each part:}}
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 | K143065 || Aad9 - Terminator || Pending
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