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Team:Warsaw/Calendar-Main/16 September 2008
From 2008.igem.org
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<h4>Piotr</h4> | <h4>Piotr</h4> | ||
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li> | <p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li> | ||
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| + | <p>Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega.</p> | ||
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| - | Inoculation to one tube (0 | + | <p>Competition tests: <br> |
| + | Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml) | ||
<ul><li>A-omega, omega and Z-omega - A_alpha protein added ~100 μg</li> | <ul><li>A-omega, omega and Z-omega - A_alpha protein added ~100 μg</li> | ||
<li>A-omega, omega and Z-omega - Z_alfa protein added ~100 μg</li> | <li>A-omega, omega and Z-omega - Z_alfa protein added ~100 μg</li> | ||
| - | <li>A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg</li> </ul> | + | <li>A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg</li> </ul></p> |
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Revision as of 22:27, 15 October 2008
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PiotrTesting of
Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega. Competition tests:
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