Team:Warsaw/Calendar-Main/17 September 2008

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<h3>Competition tests</h3>
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<h4>Piotr</h4>
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<p><ol><li>Plasmid isolation from previous day's cultures.</li><li>Digest with SacI and BamHI.</li><li>Electrophoresis.</li></ol></p>
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[photo of the gel is to be placed here]
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<p>Conclusion: cells with interracting protein survive competition!</p>
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Testy na konkurencje
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<h3>MutD5 testing</h3>
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Piotr
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<h4>Piotr</h4>
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Izolacja plazmidów z chodowli z poprzedniego dnia, cięcie saci i bamhi, elektroforeza
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<p>Inoculation of MutD5 into medium with tetracycline.</p>
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(zdjęcie żelu)
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komórki mające białko oddziałujące wygrywają konkurencję!!!
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Testy na MutD5: Piotr
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zaszczepienie MutD5 do pożywki z Tc
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<h3>Optimisation of primers for preparation of parts</h3>
<h3>Optimisation of primers for preparation of parts</h3>
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<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=http://www.fermentas.com/techinfo/nucleicacids/mappuc1819.htm>pUC19</a> plasmid using
<li><a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a> on <a href=http://www.fermentas.com/techinfo/nucleicacids/mappuc1819.htm>pUC19</a> plasmid using
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
<a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaL_link">OmegaL_link</a> and <a href="https://2008.igem.org/Wiki/Team:Warsaw/primers#OmegaP_EPB">OmegaP_EPB</a>
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primers (elongation length 30s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig4">Fig. 4</a>.</li>
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primers (elongation length 30 s). <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig3">Fig. 3</a> and <a href="https://2008.igem.org/Team:Warsaw/Calendar-Main/13_June_2008#fig4">Fig. 4</a>.</li>
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<p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 degrees.</p>
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<p>Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 &deg;C.</p>
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Revision as of 22:38, 15 October 2008

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Competition tests

Piotr

  1. Plasmid isolation from previous day's cultures.
  2. Digest with SacI and BamHI.
  3. Electrophoresis.

[photo of the gel is to be placed here]

Conclusion: cells with interracting protein survive competition!

MutD5 testing

Piotr

Inoculation of MutD5 into medium with tetracycline.

Optimisation of primers for preparation of parts

Michał K.

  1. PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using AL_BNXNE and AP+XhoI+SpeI primers (elongation length 45s).
  2. PCR on pACYC177+OmpA_omega_deltaA_alpha plasmid using LinLSXNE and AlfaPSpe primers (elongation length 45s). Fig. 2 and Fig. 3.
  3. PCR on pUC19 plasmid using OmegaL_link and OmegaP_EPB primers (elongation length 30 s). Fig. 3 and Fig. 4.

    4. Each reaction was carried out with 15 and 20 cycles and in temperature gradient from 55 to 75 °C.

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