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Team:Warsaw/Calendar-Main/30 June 2008
From 2008.igem.org
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| + | <h3>Change of the reporter from pZC with B-galactosidaze to GFP or RFP</h3> | ||
| + | <h4>Piotr, Weronika</h4> | ||
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| + | <li>There were blue and white colonies but no red or green in UV</li> | ||
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| + | <li>Inoculation of liquid LB medium + Amp with 5 white colonies | ||
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| + | </li></ol> | ||
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<h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | <h3>Cloning alpha-A and omega-A fusions on <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pKSII%2B>pKS</a></h3> | ||
<h4>Michał L., Ewa, Marcin</h4> | <h4>Michał L., Ewa, Marcin</h4> | ||
Revision as of 09:00, 16 October 2008
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Change of the reporter from pZC with B-galactosidaze to GFP or RFPPiotr, Weronika
Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinInoculation of liquid LB medium with 6 transformant colonies. Preparation of constructs with OmpA protein fusionsPawełInoculation of E. coli with pET15b plasmid on LB + ampicillin.
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