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Team:Warsaw/Calendar-Main/30 June 2008
From 2008.igem.org
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<li>There were blue and white colonies but no red or green in UV</li> | <li>There were blue and white colonies but no red or green in UV</li> | ||
| - | <li>Inoculation of liquid LB medium + Amp with | + | <li>Inoculation of liquid LB medium + Amp with white colonies</li> |
<li>Inoculation of 10ml LB with E.coli TOP10 carrying pZC</li> | <li>Inoculation of 10ml LB with E.coli TOP10 carrying pZC</li> | ||
<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator) | <li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator) | ||
Revision as of 09:25, 16 October 2008
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Change of the reporter from pZC with B-galactosidaze to GFP or RFPPiotr, Weronika
Cloning alpha-A and omega-A fusions on pKSMichał L., Ewa, MarcinInoculation of liquid LB medium with 6 transformant colonies. Preparation of constructs with OmpA protein fusionsPawełInoculation of E. coli with pET15b plasmid on LB + ampicillin.
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