Team:Warsaw/Calendar-Main/4 July 2008

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<h3>Changing of the reporter from pZC with B-galactosidaze to GFP or RFP</h3>
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<h4>Piotr, Weronika</h4>
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<li><a href="https://2008.igem.org/Wiki/Team:Warsaw/protocols#plasmid_DNA_isolation">Isolation</a> of plasmids from transformants.</li>
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<li>Control <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#digest"> digest</a> of plasmids with NotI.</li>
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<li>Gel electrophoresis of digested DNA there where proper plasmids, but they weren't red or green in UV (the reason might be low number of plasmid copies or something else)</li>
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No sucess in changing of the reporter from pZC with B-galactosidaze to GFP or RFP
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<h3>Preparation of constructs with OmpA protein fusions</h3>
<h3>Preparation of constructs with OmpA protein fusions</h3>
<h4>Paweł</h4>
<h4>Paweł</h4>

Revision as of 09:43, 16 October 2008

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Changing of the reporter from pZC with B-galactosidaze to GFP or RFP

Piotr, Weronika

  1. Isolation of plasmids from transformants.
  2. Control digest of plasmids with NotI.
  3. Gel electrophoresis of digested DNA there where proper plasmids, but they weren't red or green in UV (the reason might be low number of plasmid copies or something else)
No sucess in changing of the reporter from pZC with B-galactosidaze to GFP or RFP

Preparation of constructs with OmpA protein fusions

Paweł

  1. Isolation of plasmids from bacterial cultures inoculated on previous day (pET15b+OmpA_alpha and pET15b+OmpA_omega).
  2. Control digest of plasmids with SacI and BamHI (BamHI buffer).
  3. Gel electrophoresis.



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