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Template:Team:UC Berkeley/Notebook/Molly sequencing
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| MEA049 || 10/14/08 || pK112245 || new #2 || JH126F/R || Both attR1 and attR2 sites look fine || [[Team:UC Berkeley/Notebook/MEA/MEA049 | MEA049]] | | MEA049 || 10/14/08 || pK112245 || new #2 || JH126F/R || Both attR1 and attR2 sites look fine || [[Team:UC Berkeley/Notebook/MEA/MEA049 | MEA049]] | ||
|- | |- | ||
| - | | MEA051 || 10/16/08 || pK112246 || new #1 || JH126F/R || Both attR1 and attR2 sites look fine || [[Team:UC Berkeley/Notebook/MEA/ | + | | MEA051 || 10/16/08 || pK112246 || new #1 || JH126F/R || Both attR1 and attR2 sites look fine || [[Team:UC Berkeley/Notebook/MEA/MEA051 | MEA051]] |
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Latest revision as of 19:04, 16 October 2008
Molly's Sequencing Log
| Read | Date | Plasmid | Clone # | Oligo | Result | File |
| MEA001 | 06/15/08 | pBca1256-K112200 | 1 | ca998 | Bad read | MEA001 |
| MA001 | 06/18/08 | pBca1256-K112200 | 2 | ca998 | Point mutation changing Asn to Lys | MA001 |
| MEA002 | 06/15/08 | pBca1256-K112201 | 1 | ca998 | Point mutation changing Asn to Lys | MEA002 |
| MA002 | 06/18/08 | pBca1256-K112201 | 2 | ca998 | Point mutation changing Asn to Lys | MA002 |
| MEA003 | 06/15/08 | pBca1256-K112202 | 1 | ca998 | Perfect | MEA003 |
| MEA004 | 06/15/08 | pBca1256-K112203 | 1 | ca998 | Perfect | MEA004 |
| MEA005 | 06/15/08 | pBca1256-K112204 | 1 | ca998 | Point mutation changing Asn to Lys | MEA005 |
| MA003 | 06/18/08 | pBca1256-K112204 | 2 | ca998 | Bad Read | MA003 |
| MEA006 | 06/15/08 | pBca1256-K112207 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA006 |
| MEA006 | 06/18/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA006 |
| AL305 | 06/23/08 | pBca1256-K112207 | 1 | G00101 | Bad reverse read, 2 templates | AL305 |
| MEA007 | 06/15/08 | pBca1256-K112208 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA007 |
| MEA007 | 06/18/08 | pBca1256-K112208 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA007 |
| MA212 | 06/23/08 | pBca1256-K112208 | 1 | G00101 | Perfect read, can see silent mutation from AGG to AGA seen in forward read | MA212 |
| MEA008 | 06/15/08 | pBca1256-K112209 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA008 |
| MEA008 | 06/18/08 | pBca1256-K112209 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA008 |
| MA209 | 06/23/08 | pBca1256-K112209 | 1 | G00101 | Perfect reverse read, able to see the silent mutation from AGG to AGA seen in forward read | MA209 |
| MEA009 | 06/15/08 | pBca1256-K112210 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA009 |
| MEA009 | 06/18/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA009 |
| MA211 | 06/23/08 | pBca1256-K112210 | 1 | G00101 | Bad reverse read, two templates present | MA211 |
| MEA010 | 06/15/08 | pBca1256-K112211 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA010 |
| MEA010 | 06/18/08 | pBca1256-K112211 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA010 |
| MA210 | 06/23/08 | pBca1256-K112211 | 1 | G00101 | Perfect reverse read, can see silent mutation from AGG to AGA seen in forward read | MA210 |
| MEA011 | 06/15/08 | pBca1256-K112212 | 1 | ca998 | Partial read (long part) with silent mutation from AGG to AGA | MEA011 |
| MEA011 | 06/18/08 | pBca1256-K112212 | 1 | G00101 | Bad reverse read, but good part was perfect with silent mutation from AGG to AGA | MEA011 |
| MA208 | 06/23/08 | pBca1256-K112212 | 1 | G00101 | Bad reverse read, two templates present | MA208 |
| MEA012 | 06/15/08 | pBca1256-K112213 | 1 | ca998 | Perfect | MEA012 |
| MEA013 | 06/15/08 | pBca1256-K112214 | 1 | ca998 | Perfect | MEA013 |
| MEA014 | 06/15/08 | pBca1256-K112215 | 1 | ca998 | Perfect | MEA014 |
| MEA015 | 06/15/08 | pBca1256-K112216 | 1 | ca998 | Bad read in the beginning, re-sequence & pick new colony | MEA015 |
| MEA015 | 06/18/08 | pBca1256-K112216 | 1 | ca998 | This one was perfect except it shows a deletion of A in EcoRI site, but that's probably just a problem with the sequencing data | MEA015 |
| MA005 | 06/18/08 | pBca1256-K112216 | 2 | ca998 | Perfect | MA005 |
| MEA016 | 06/15/08 | pBca1256-K112217 | 1 | ca998 | Perfect match to K112218 | MEA016 |
| MEA016 | 06/18/08 | pBca1256-K112217 | 1 | ca998 | Perfect match to K112218 | MEA016 |
| MEA017 | 06/15/08 | pBca1256-K112218 | 1 | ca998 | Perfect match to K112217 | MEA017 |
| MEA017 | 06/18/08 | pBca1256-K112218 | 1 | ca998 | Perfect match to K112217 | MEA017 |
| MEA219 | 06/20/08 | pBca1256-K1122219 | 1 | ca998 | Perfect | MEA219 |
| MEA220 | 06/20/08 | pBca1256-K112220 | 1 | ca998 | Perfect, but there's an additional BamHI site inserted after the first one... | MEA220 |
| MEA018 | 06/15/08 | pBca1256-K112221 | 1 | ca998 | Perfect | MEA018 |
| MEA019 | 06/15/08 | pBca1256-K112222 | 1 | ca998 | Perfect | MEA019 |
| MEA223 | 06/20/08 | pBca1256-K112223 | 1 | ca998 | Perfect | MEA223 |
| MEA224 | 06/20/08 | pBca1256-K112224 | 1 | ca998 | Perfect | MEA224 |
| MEA020 | 06/15/08 | pBca1256-K112225 | 1 | ca998 | Perfect | MEA020 |
| MEA021 | 06/15/08 | pBca1256-K112226 | 1 | ca998 | Perfect | MEA021 |
| MEA022 | 06/15/08 | pBca1256-K112227 | 1 | ca998 | Perfect | MEA022 |
| MEA023 | 06/15/08 | pBca1256-K112228 | 1 | ca998 | Perfect match to K112229 | MEA023 |
| MEA024 | 06/15/08 | pBca1256-K112229 | 1 | ca998 | Bad read, re-sequence | MEA024 |
| MA004 | 06/18/08 | pBca1256-K112229 | 2 | ca998 | Insertion of an A... pick a new colony | MA004 |
| DV5 | 06/20/08 | pBca1256-K112229 | 3 | ca998 | Perfect match to K112230 (aside from a deletion of an A in the EcoRI site... probably just due to bad reading. Don't worry about it) | DV5 |
| MEA025 | 06/15/08 | pBca1256-K112230 | 1 | ca998 | Perfect match to K112228 | MEA025 |
| MEA026 | 06/24/08 | pBca1256-K112200 | 1 | ca998 | Perfect | MEA026 |
| MEA027 | 06/24/08 | pBca1256-K112201 | 1 | ca998 | Fatal point mutation from GCG to GTG (Ala to Val) | MEA027 |
| MEA028 | 06/24/08 | pBca1256-K112204 | 1 | ca998 | Mutation in a~ region from tgcg to tgtg, and fatal deletion of G. | MEA028 |
| MEA029 | 06/24/08 | pBca1256-K112205 | 1 | ca998 | Perfect | MEA029 |
| MEA030 | 06/24/08 | pBca1256-K112207 | 1 | g00101 | Perfect | MEA030 |
| MEA031 | 06/24/08 | pBca1256-K112210 | 1 | g00101 | Perfect | MEA031 |
| MEA032 | 06/24/08 | pBca1256-K112212 | 1 | g00101 | Perfect | MEA032 |
| MEA033 | 06/26/08 | pBca1256-K112201 | 2 | ca998 | Perfect | MEA033 |
| MEA034 | 06/26/08 | pBca1256-K112201 | 3 | ca998 | Deletion of GCGATGT | MEA034 |
| MEA035 | 06/26/08 | pBca1256-K112204 | 2 | ca998 | Perfect | MEA035 |
| MEA036 | 06/26/08 | pBca1256-K112204 | 3 | ca998 | Insertion of GCGATGT | MEA036 |
| Molly6 | 07/22/08 | pBjh1601KA-K112208 | 2 | ca998 | Perfect | Molly6_ca998 |
| Molly6 | 07/22/08 | pBjh1601KA-K112208 | 2 | G00101 | Perfect | Molly6_G00101 |
| Molly7 | 07/22/08 | pBjh1601AC-K112230 | 2 | ca998 | Sequenced perfectly for a~pelB (K112229) | Molly7 |
| Molly13-2 | 07/23/08 | pBjh1601KA-K112235 | 2 | ca998 | Perfect | Molly13-2_ca998 |
| Molly13-2 | 07/23/08 | pBjh1601KA-K112235 | 2 | G00101 | Perfect | Molly13-2_G00101 |
| Molly15-1 | 07/23/08 | pBjh1601AK-{rbs.ompT}{<int!} | 1 | ca998 | Perfect | Molly15-1 |
| Molly18-5 | 07/23/08 | pBjh1601CA-{rbs.phoApp}{<ihfB!} | 5 | ca998 | Perfect, but then we realized the wrong part was made initially. | Molly18-5 |
| Molly17-5 | 07/24/08 | pBjh1601KC-{rbs.lamB}{<ihfA!} | 5 | ca998 | Perfect, but then we realized the wrong part was made initially. | Molly17-5 |
| ig9 | 07/25/08 | pBjh1601AK-K112205 | 1 | ca998 | Perfect | ig9 |
| Molly2-2 | 07/25/08 | pBjh1601CA-K112212 | 2 | ca998 | Perfect sequence to a~int! (K112211) | Molly2-2 |
| Molly#3 | 07/26/08 | pBjh1601AK-K112236 | 3 | ca998 | Perfect, shows deletion in Kan part of vector | Molly#3 |
| 234-CK-1 | 08/05/08 | pBjh1601CK-K112234 | 1 | ca998 | Perfect | 234-CK-1_ca998 |
| 234-CK-1 | 08/05/08 | pBjh1601CK-K112234 | 1 | G00101 | G00101 site deleted in Kan part of vector, bad read | 234-CK-1_G00101 |
| 234-CK-2 | 08/05/08 | pBjh1601CK-K112234 | 2 | ca998 | Perfect | 234-CK-2_ca998 |
| 234-CK-2 | 08/05/08 | pBjh1601CK-K112234 | 2 | G00101 | G00101 site deleted in Kan part of vector, bad read | 234-CK-2_G00101 |
| 234-1256-3 | 08/08/08 | pBca1256-K112234 | 3 | ca998 | Perfect | 234-1256-3_ca998 |
| 234-1256-3 | 08/08/08 | pBca1256-K112234 | 3 | G00101 | Perfect | 234-1256-3_G00101 |
| 237-CA-1 | 08/08/08 | pBjh1601CA-K112237 | 1 | mea010 | Perfect | 237-CA-1_mea010 |
| MEA037 | 10/07/08 | pK112247 | 1 | ca1246F/R | AttL1 looks good, but attL2 looks like it might have a mutation from G->A... | MEA037 |
| MEA038 | 10/07/08 | pK112247 | 2 | ca1246F/R | Both attL1 and attL2 sites look good | MEA038 |
| MEA039 | 10/07/08 | pK112248 | 1 | ca1246F/R | Both attL1 and attL2 sites look good | MEA039 |
| MEA040 | 10/07/08 | pK112248 | 2 | ca1246F/R | Both attL1 and attL2 sites look good | MEA040 |
| MEA041 | 10/07/08 | pK112245 | 3 | JH126F | attR1 site looks good | MEA041 |
| MEA042 | 10/07/08 | pK112245 | 4 | JH126F | attR1 site looks good | MEA042 |
| MEA043 | 10/07/08 | pK112246 | 1 | JH126F | attR1 site looks good | MEA043 |
| MEA044 | 10/07/08 | pk112246 | 5 | JH126F | attR1 site looks good | MEA044 |
| MEA045 | 10/10/08 | pK112128 | 1 | JH126R | AttR2 site looks fine | MEA045 |
| MEA046 | 10/10/08 | pK112245 | 4 | JH126R | Mutation in attR2 site | MEA046 |
| MEA047 | 10/10/08 | pK112246 | 5 | JH126R | Mutation in attR2 site | MEA047 |
| MEA048 | 10/10/08 | pK112128 | 2 | JH126R | AttR2 site looks fine | MEA048 |
| MEA049 | 10/14/08 | pK112245 | new #2 | JH126F/R | Both attR1 and attR2 sites look fine | MEA049 |
| MEA051 | 10/16/08 | pK112246 | new #1 | JH126F/R | Both attR1 and attR2 sites look fine | MEA051 |
