Project

From 2008.igem.org

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    <td height="32" colspan="2"><span class="STYLE3">Design</span></td>
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    <td width="497" rowspan="3">&nbsp;</td>
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    <td width="502" height="108"><p class="STYLE4">The list of the ideas generated from the  brainstorm<br />
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      The paper-making E coli<br />
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      A time relay device (E coli baby sitter) <br />
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      An E coli Pipeline <br />
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slideimages[0]='<a href="index.htm"><img src="第一张图片.gif" border=0"></a>'
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      Torch rely <br />
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slideimages[1]='<a href="index1.htm"><img src="第二张图片.gif"></a>'
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      Tumor-killer <br />
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slideimages[2]='<a href="index2.htm"><img src="第三张图片.gif"></a>'
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      E coli neural pathway<br />
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slideimages[3]='<a href="index3.htm"><img src="第四张图片.gif"></a>'
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    …….</p></td>
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    <td><p class="STYLE4">The coexistence of organisms has been in  the focus of research interests for a while, revealing the interactions between  species not only contributes to the deeper understanding of natural systems but  also provides perspectives for more efficient coculture of organisms that  function in pharmaceutical engineering and soil remediation.</p>
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    <p class="STYLE4">As in prisoners’ dilemma, the bacteria in  our design are faced with two solutions for coexistence, they could either  choose to cooperate with one another by providing inducers to express their  partners’ antibiotics-resistance genes or they could take a foe strategy in  which no cooperation is needed for both strains’ survival.</p></td>
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    <td><p class="STYLE4">To realize this goal, toggle switches have  been built to regulate the interactions between the two strains. As is shown in  the illustration above, we used kanamycin  and&nbsp;chloramphenicol&nbsp;as the selective forces. </p>
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      <p class="STYLE4">When  auto-inducers, either AHL or BHL is introduced to the culture, LuxPr or Prhl  will be activated to produce the auto-inducers required by their partners, and  express the anti-biotic resistant genes to ensure each other’s survival. During  the process, PBad/arac and Plac will be repressed because of the repressors  Arac and LacI expressed by LuxPr and PrhI. <br />
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    However, upon the introduction of IPTG or arobinose, the repressors will stop  functioning, so that Aiia will be expressed, and therefore AHL and BHL will be  degraded, so that there will be no communications any more, and the  relationship between the two strains will enter the phase of competition. </p></td>
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    <td height="17" colspan="2"><span class="STYLE3">Model</span></td>
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    <td height="17" colspan="2"><span class="STYLE3">Experiment</span></td>
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Revision as of 09:56, 19 October 2008

无标题文档

Design
 

The list of the ideas generated from the brainstorm
The paper-making E coli
A time relay device (E coli baby sitter)
An E coli Pipeline
Torch rely
Tumor-killer
E coli neural pathway
…….

The coexistence of organisms has been in the focus of research interests for a while, revealing the interactions between species not only contributes to the deeper understanding of natural systems but also provides perspectives for more efficient coculture of organisms that function in pharmaceutical engineering and soil remediation.

As in prisoners’ dilemma, the bacteria in our design are faced with two solutions for coexistence, they could either choose to cooperate with one another by providing inducers to express their partners’ antibiotics-resistance genes or they could take a foe strategy in which no cooperation is needed for both strains’ survival.

To realize this goal, toggle switches have been built to regulate the interactions between the two strains. As is shown in the illustration above, we used kanamycin and chloramphenicol as the selective forces.

When auto-inducers, either AHL or BHL is introduced to the culture, LuxPr or Prhl will be activated to produce the auto-inducers required by their partners, and express the anti-biotic resistant genes to ensure each other’s survival. During the process, PBad/arac and Plac will be repressed because of the repressors Arac and LacI expressed by LuxPr and PrhI.
However, upon the introduction of IPTG or arobinose, the repressors will stop functioning, so that Aiia will be expressed, and therefore AHL and BHL will be degraded, so that there will be no communications any more, and the relationship between the two strains will enter the phase of competition.

Model
 
Experiment

 

 

 

 

Retrieved from "http://2008.igem.org/Project"