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Team:Warsaw/Calendar-Main/23 June 2008
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| - | There is no mutation in | + | The analysis of lacZ sequence from pZC<br> |
| - | This is wery strange... We must make new reporter system. | + | There is no mutation in lacZ gene. Why the colonys are white??? Reason of white phenotype unknown (thisis must be chromosomal mutation, transcription supresion or something else) |
| + | This is wery strange... We must make new reporter system... | ||
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PiotrThe analysis of lacZ sequence from pZCThere is no mutation in lacZ gene. Why the colonys are white??? Reason of white phenotype unknown (thisis must be chromosomal mutation, transcription supresion or something else) This is wery strange... We must make new reporter system... Preparation of constructs with OmpA protein fusionsMichał K.Inoculation other separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha and pACYC177+OmpA_omega (liquid LB + kanamycin). Preparation of alpha-A and omega-A fusionsMichał L.We have successfully amplified Omega-linker (Fig. 1), but were unable to obtain the other. two PCRs were repeated. New annealing temp.: 48°C. Mg2+ concentration elevated to 4 mM. 
Fig. 1.PCR products. Lane 1 - lack of product for A-linker; lane 3 - product for Omega-linker.
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