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Team:Warsaw/Calendar-Main/19 June 2008
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<p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | <p> Inoculation of separate transformant colonies from plates with ligations: <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-alpha>pACYC177+OmpA_alpha</a> <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pACYC177%2BompA-omega>pACYC177+OmpA_omega</a> (liquid LB + kanamycin).</p> | ||
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There is no mutation in beta-galactosidase gene. Why the colonys are white??? | There is no mutation in beta-galactosidase gene. Why the colonys are white??? | ||
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| - | + | There is no mutation in beta-galactosidase gene. Why the colonys are white???</li> | |
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<li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | <li>Colony <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#pcr">PCR</a>.<br> | ||
Revision as of 18:35, 19 October 2008
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Preparation of constructs with OmpA protein fusionsMichał K.Inoculation of separate transformant colonies from plates with ligations: pACYC177+OmpA_alpha pACYC177+OmpA_omega (liquid LB + kanamycin). There is no mutation in beta-galactosidase gene. Why the colonys are white???There is no mutation in beta-galactosidase gene. Why the colonys are white??? Template: DNA isolated from white colonies Primers: pZCseqL and pZCseqR
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