Team:Warsaw/Calendar-Main/27 August 2008

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<h3>Cloning of protein Z DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Antoni</h4>
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<p> Transformant colonies inoculated to liquid LB with ampicillin.</p>
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<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4>
<h3>Cloning of protein A DNA to <A href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BOmpA-alpha>pET15b+OmpA-alpha</a> plasmid</h3><h4>Michał K.</h4>

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Cloning of protein A DNA to pET15b+OmpA-alpha plasmid

Michał K.

  1. Digest of pGeneart+A and pET15b+Omp-alpha with NdeI and NotI (Orange buffer), pET15b was also dephosphorylated.
  2. Gel electrophoresis of digested plasmids and gel-out of proper bands (400 bp for A lane and 6200 bp for pET15b lane).
  3. Ligation of pET15b+alpha and A.
  4. Transformation of E. coli TOP10.
  5. Transformants plating on LB + ampicillin.

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