Team:Warsaw/Calendar-Main/29 August 2008

From 2008.igem.org

(Difference between revisions)
Line 9: Line 9:
-
<h3>Protein purification: A-alpha, Z-alpha i Z-omega</h3>
+
<h3>Protein purification: A-alpha</h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
<p>
<p>
-
Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+A-alpha, pET15b+Z-alpha and pET15b+Z-omega. Plating them on LB with chloramphenicol and ampicillin.</p></html>
+
Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.</p></html>

Revision as of 20:53, 19 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Cloning of protein A DNA to pET15b+Omp-alpha plasmid

Michał K.

  1. Isolation of pET15b+A-alpha plasmids.
  2. Control digest of isolated plasmids with NdeI and NotI (Orange buffer).
  3. Gel elctrophoresis. Chioce of proper clone.

Protein purification: A-alpha

Piotr

Single transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_August_2008"