Team:Warsaw/Calendar-Main/4 September 2008

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<h3>Purification of proteins:  A-alpha, Z-alpha and Z-omega</h3><h4>Piotr, Emilia</h4>
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<h3>Purification of proteins:  A-alpha</h3><h4>Piotr, Emilia</h4>
<p>100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</p>
<p>100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to <a href=https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Z_a_Z_o>protocol</a>).</p>

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Purification of proteins: A-alpha

Piotr, Emilia

100 ml of medium inoculated with overnight culture, then used as inoculum for 1 l culture with presence of ampicillin, kanamycin and IPTG (according to protocol).