Waterloo/9 September 2008

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Revision as of 19:21, 20 October 2008

080909 Tue

[x] check crossover PCR primers

Parts being transferred to a BioBrick vector: [x] colony PCR on transformants of ligation mixes [ERICA] [x] run products on a gel (will probably take 2 gels) [SOMIL]

[x] kit prep of parts for sequencing (prep only ONE tube from each pair of duplicates) [SOMIL HILLARY] [x] make glycerol stocks

[x] send above pDNA for sequencing [SHIRA]

print lab handbook for [x] Somil [] Steve

[x] reorganize freezer boxes [DANIELLE and HILLARY] (did enzyme/PCR boxes)

@@ Line 1: / Line 1: @@
-<u>080908 Mon</u><br>
+Tue
-[x] check Sunday's transformation results<br>
+[x] check crossover PCR primers
-Putative good constructs yet to be sequenced:<br>
+Parts being transferred to a BioBrick vector:
-[x] inoculate culture from transformants of parts for sequencing (and making glycerol stock)<br>
+[x] colony PCR on transformants of ligation mixes [ERICA]
-[x] inoculate culture from transformants of synthesized parts in original GeneArt vector (for glycerol stock)<br>
+[x] run products on a gel (will probably take 2 gels) [SOMIL]
-[x] save glycerol stock of Plac-rfp"<br>
+[x] kit prep of parts for sequencing (prep only ONE tube from each pair of duplicates) [SOMIL HILLARY]
+[x] make glycerol stocks
-[x] miniprep pSB2K3(gene6) and pSB2K3(PmeI) for diag digest and gel<br>
+[x] send above pDNA for sequencing [SHIRA]
-[x] digest of above miniprep<br>
-[x] gel of above digest<br>
-[x] for each part, subculture one correct-looking sample in duplicate to kit prep and save stock of tomorrow<br>
-[] design crossover PCR primers<br>
+print lab handbook for
-- Half done<br>
+[x] Somil
+[] Steve
-[x] pick time for lab meeting<br>
+[x] reorganize freezer boxes [DANIELLE and HILLARY] (did enzyme/PCR boxes)
-- Meeting has been set for Wednesday Sept. 10th at 5:30<br>