"
Team:Paris/Modeling/f5
From 2008.igem.org
(Difference between revisions)
| Line 57: | Line 57: | ||
|comments | |comments | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>52</sub> |
| - | |production rate of | + | |production rate of FlhDC-pFliL '''with RBS E0032''' <br> β<sub>52</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>46</sub>/{coef<sub>fliA</sub>}) |
| - | |activation constant of | + | |activation constant of FlhDC-pFliL <br> K<sub>46</sub> |
|nM | |nM | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>46</sub> |
| - | |complexation order of | + | |complexation order of FlhDC-pFliL <br> n<sub>46</sub> |
|no dimension | |no dimension | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |β<sub> | + | |β<sub>53</sub> |
| - | |production rate of | + | |production rate of FliA-pFliL '''with RBS E0032''' <br> β<sub>53</sub> |
|nM.min<sup>-1</sup> | |nM.min<sup>-1</sup> | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |(K<sub> | + | |(K<sub>47</sub>/{coef<sub>omp</sub>}) |
| - | |activation constant of | + | |activation constant of FliA-pFliL <br> K<sub>47</sub> |
|nM | |nM | ||
| | | | ||
| | | | ||
|- | |- | ||
| - | |n<sub> | + | |n<sub>47</sub> |
| - | |complexation order of | + | |complexation order of FliA-pFliL <br> n<sub>47</sub> |
|no dimension | |no dimension | ||
| | | | ||
Revision as of 22:33, 20 October 2008
We have [FlhDC] = {coefflhDC}expr(pTet) = {coefflhDC} ƒ1([aTc]i)
and [FliA] = {coefFliA}expr(pBad) = {coefFliA} ƒ2([arab]i)
So, at steady-states,
| param | signification | unit | value | comments |
| [expr(pFlhDC)] | expression rate of pFlhDC with RBS E0032 | nM.min-1 | need for 20 mesures with well choosen values of [aTc]i and for 20 mesures with well choosen values of [arab]i and 5x5 measures for the relation below? | |
| γGFP | dilution-degradation rate of GFP(mut3b) | min-1 | 0.0198 | |
| [GFP] | GFP concentration at steady-state | nM | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| (fluorescence) | value of the observed fluorescence | au | need for 20 + 20 measures and 5x5 measures for the relation below? | |
| conversion | conversion ratio between fluorescence and concentration | nM.au-1 | (1/79.429) |
| param | signification corresponding parameters in the equations | unit | value | comments |
| β52 | production rate of FlhDC-pFliL with RBS E0032 β52 | nM.min-1 | ||
| (K46/{coeffliA}) | activation constant of FlhDC-pFliL K46 | nM | ||
| n46 | complexation order of FlhDC-pFliL n46 | no dimension | ||
| β53 | production rate of FliA-pFliL with RBS E0032 β53 | nM.min-1 | ||
| (K47/{coefomp}) | activation constant of FliA-pFliL K47 | nM | ||
| n47 | complexation order of FliA-pFliL n47 | no dimension |
Then, if we have time, we want to verify the expected relation
