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Materials and Methods
From 2008.igem.org
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| + | In brief: yeast genomic DNA was used as a template to PCR amplify "parts". Parts were TOPO cloned, validated by sequencing, and then combinatorially cloned into acceptor vectors: | ||
Revision as of 16:22, 22 October 2008
Materials and Methods
1. Cloning
Constructs were cloned using the AarI method, developed by Sergio Pesajovich for the 2007 iGEM competition. To learn more about AarI cloning:
Everything you ever wanted to know about AarI
In brief: yeast genomic DNA was used as a template to PCR amplify "parts". Parts were TOPO cloned, validated by sequencing, and then combinatorially cloned into acceptor vectors:
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