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1. Making a DNA agarose gel
From 2008.igem.org
(Difference between revisions)
(New page: Making the Agarose Gel (for a 1%gel 50mL volume) 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. 2. Thoroughly mix the two in the flask and microwave the mixture for ...) |
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| - | + | 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder.<br><br> | |
| - | + | 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).<br><br> | |
| - | 1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder. | + | 3. Add 1.5µL of Ethimium Bromide and swirl until a fine mixture is seen.<br><br> |
| - | 2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling). | + | 4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.<br><br> |
| - | 3. Add 1.5µL of Ethimium Bromide and swirl until a fine mixture is seen. | + | 5. Wait for the gel to solidify over time.<br><br> |
| - | 4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends. | + | 6. Once hardened, remove the comb and load the gel into the electrophoresis box.<br><br> |
| - | 5. Wait for the gel to solidify over time. | + | |
| - | 6. Once hardened, remove the comb and load the gel into the electrophoresis box. | + | |
Latest revision as of 20:02, 13 June 2008
1. Weigh out 50mL of TAE buffer and mix with 0.5g of Agarose powder.
2. Thoroughly mix the two in the flask and microwave the mixture for roughly about 1 minute (or until you see bubbling).
3. Add 1.5µL of Ethimium Bromide and swirl until a fine mixture is seen.
4. Pour the mixture onto the casting tray (make sure that each end is taped), with a comb inserted in one of the ends.
5. Wait for the gel to solidify over time.
6. Once hardened, remove the comb and load the gel into the electrophoresis box.
