Team:UCSF/Willis Wong Notebook
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Revision as of 07:04, 23 October 2008
Willis Wong Notebook
Overall Objective:
Devise an array of inputs that cab be linked to regulation of silencing.
Milestones:
1. Define minimal fragment for light inducible dimerization. 2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing / Unsilencing)
Work Done:
Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts). Week 3-4: Ligate AB + BD parts into 315 vectors. Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)]. Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3. Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.