Team:Warsaw/Calendar-Main/16 September 2008

From 2008.igem.org

(Difference between revisions)
Line 2: Line 2:
<!-- do not edit above me! -->
<!-- do not edit above me! -->
<html>
<html>
 +
<h3>'Hunter and prey' system tests</h3>
<h4>Piotr, Emilia</h4>
<h4>Piotr, Emilia</h4>
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>

Revision as of 10:51, 23 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


'Hunter and prey' system tests

Piotr, Emilia

Testing of

  • resistance to antibiotics achieved by interacting with a protein in medium;
  • minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;

Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega.

Competition tests:
Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  • A-omega, omega and Z-omega - A_alpha protein added ~100 μg
  • A-omega, omega and Z-omega - Z_alfa protein added ~100 μg
  • A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/16_September_2008"