Team:Warsaw/Calendar-Main/29 May 2008

From 2008.igem.org

(Difference between revisions)
Line 19: Line 19:
</ol></p>
</ol></p>
-
 
+
<h3></h3>
<h4>Piotr</h4>
<h4>Piotr</h4>
<p>OD measurement of above cultures.</p>
<p>OD measurement of above cultures.</p>

Revision as of 10:54, 23 October 2008

Gallery Bricks Notebook Team Project Home


Previous day
return to main notebook page
Previous entry
Interactive list of topics
next notebook entry

 


Rifampicin test

Michał L., Ewa, Marcin

  1. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB.
  2. Added 50 μL E. coli TOP10 carrying pMPM-T5+AID (in liquid LB) into liquid LB + 0.02% arabinose.
  3. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB.
  4. Added 50 μL E. coli TOP10 carrying pTrc99A+AID (in liquid LB) into liquid LB + 1 mM IPTG.
  5. Waiting 5 hours.
  6. 1 ml (2x) from p.1, p.2, p.3, p.4 plating on LB + rifampicin.

Piotr

OD measurement of above cultures.

Retrieved from "http://2008.igem.org/Team:Warsaw/Calendar-Main/29_May_2008"