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Revision as of 13:00, 23 October 2008
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BioBricks & Characterisation
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Welcome to our Biobricks and Characterisation page! Here you can find information about the parts, devices and systems we have produced.
Shown below is our final construct - our light-sensing, clutch-engaging, biomaterial-producing biofabricator. If you move your mouse over a part, it will light up and you can click it to visit its page in the iGEM Registry. If you select the name of a device (or the whole system) underneath the diagram, that device will show up and clicking the name will take you to its charaterisation page.
| Device 1 | Device 2 | Device 3 |
| The System |
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| Parts Submitted to the Registry
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For now, here are the parts we will submit to the Registry. They can also be accessed here.
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| Production Status
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This table shows our progress with each part:
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| Key
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| Submitted
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| Sequenced
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| Midiprepped
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| Fully verified
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| Partially Verified
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| Transformed
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| Ligation pending/Failed
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| Awaiting GeneArt
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| Cloning difficulties
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| Part Number | Part Name | Status
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| K143001 | 5' AmyE Integration Sequence | Sequenced
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| K143002 | 3' AmyE Integration Sequence | Sequenced
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| K143005 | 5' EpsE Integration Sequence | PCR Verified
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| K143006 | 3' EpsE Integration Sequence | Sent for Sequencing
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| K143008 | 5' PyrD Integration Sequence | Pending
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| K143009 | 3' PyrD Integration Sequence | PCR verified
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| K143031 | Aad9 Spectinomycin Resistance Gene | Sent for Sequencing
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| K143032 | EpsE Molecular Clutch Gene of B. subtilis | Sequenced
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| K143033 | LacI (Lva-, N terminal deletion) | Sequenced
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| K143034 | LipA-EAK16 Fusion | Partially Sequenced
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| K143035 | LipA-Human Elastin Fusion | Transformed
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| K143036 | Xylose Operon Regulatory Protein | PCR verified
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| K143037 | Ytva Blue Light Receptor of B. subtilis | PCR verified
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| K143038 | SacB-EAK16 Fusion | Sequenced
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| K143039 | SacB-Human Elastin Fusion | Fails to clone
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| K143050 | P43-gsiB | Sent for Sequencing
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| K143051 | P43-spoVG | Sequenced
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| K143052 | Pveg-gsiB | Sequenced
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| K143053 | Pveg-spoVG | Sequenced
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| K143054 | Phyperspank-gsiB | Pending
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| K143055 | Phyperspank-spoVG | PCR verified
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| K143056 | Pxyl-gsiB | Pending
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| K143057 | Pxyl-spoVG | Pending
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| K143058 | Pctc-gsiB | Midiprepped
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| K143059 | Pctc-spoVG | Midiprepped
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| K143060 | PgsiB-gsiB | Wrong insert cloned
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| K143061 | PgsiB-spoVG | Sequenced
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| K143062 | LacI - Terminator | Pending
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| K143063 | XylR - Terminator | Pending
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| K143064 | CAT - Terminator | Sent for Sequencing
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| K143065 | Aad9 - Terminator | Pending
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