Team:UCSF/Willis Wong Notebook

From 2008.igem.org

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Willis Wong  
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= Willis Wong =
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Notebook
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== Notebook ==
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== Overall Objective ==
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=== Overall Objective ===
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    Devise an array of inputs that can be linked to regulation of silencing.
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Devise an array of inputs that can be linked to regulation of silencing.
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== Milestones ==
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=== Milestones ===
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  1. Define minimal fragment for light inducible dimerization.
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1. Define minimal fragment for light inducible dimerization.
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  2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing /
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      Unsilencing)
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2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing/Unsilencing)
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== Work Done ==
 
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Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts).
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=== Work Done ===
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          Week 3-4: Ligate AB + BD parts into 315 vectors.
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Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts).
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          Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)].
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Week 3-4: Ligate AB + BD parts into 315 vectors.
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          Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3.
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Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)].
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          Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.
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Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3.
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Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.

Revision as of 16:28, 23 October 2008

Contents

Willis Wong

Notebook

Overall Objective

Devise an array of inputs that can be linked to regulation of silencing.

Milestones

1. Define minimal fragment for light inducible dimerization.

2. Define the configuration of dimerization/regulatory domains that will yield desired function. (Silencing/Unsilencing)


Work Done

Week 1-2: Design AB and BD parts for combinatorial cloning (includes PhyB and Pif3 parts). Week 3-4: Ligate AB + BD parts into 315 vectors. Week 4-5: Subclone Pif3 constructs into 303 vectors for intregration into a yeast reporter strain [B3(H)]. Week 7-9: Transform the PhyB constructs into the yeast strains that were integrated with Pif3. Week 10-11: In the dark, added PCB, pulsed with respective light, ran FACs.

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