Team:Chiba/Calendar-Home/14 September 2008

From 2008.igem.org

(Difference between revisions)
(New page: >Home | Notebook 13 September 2008 <|[[Team:Chiba/Calendar-Home/15 September 2008|> 15 September 2008...)
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==Laboratory work==
==Laboratory work==
===Team:Communication===
===Team:Communication===
 +
:(13/9)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]'''
 +
 +
 +
 +
:'''[[Team:Chiba/protocol/transformation|Transformation]]'''
 +
:Competent cells : XL10G
 +
::*BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
 +
::*Plac+RhlI+LVA
 +
::*Plac+CinI+LVA
 +
 +
:--->(15/9)
 +
===Team:Output===
===Team:Output===
 +
<br>[[Team:Chiba/jk/γ/Trl|Time Responce Liquid]]
 +
<br>Digestion test
 +
*[http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
 +
*[http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
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*[http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
 +
*[http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
 +
*[http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
 +
*[http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
 +
*[http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
 +
*BBa_I716542
 +
*[http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
 +
*[http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
 +
*[http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
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*[http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
 +
*[http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
 +
*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
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*[http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
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*[http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
 +
*[http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
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*[http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
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*[http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
 +
 +
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
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<td width="257">Sample No</td>
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<td>each</td>
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</tr>
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<tr>
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<td>DNA(digested sample)</td>
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<td>3</td>
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</tr>
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<tr>
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<td>Buffer2(x10)</td>
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<td>1</td>
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</tr>
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<tr>
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<td>dH2O</td>
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<td>6</td>
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</tr>
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<tr>
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<td>EcoRⅠ</td>
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<td>0.075</td>
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</tr>
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<tr>
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<td>TOTAL</td>
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<td>10</td>
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</tr>
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</table>
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-->37℃ 1hour
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 +
[[Team:Chiba/protocol/gelcheck|Gel check]]
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 +
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
 +
<tr>
 +
<td width="257">Sample No</td>
 +
<td>each</td>
 +
</tr>
 +
<tr>
 +
<td>DNA(digestion sample)</td>
 +
<td>10</td>
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</tr>
 +
<tr>
 +
<td>Loading Dye</td>
 +
<td>2</td>
 +
</tr>
 +
<tr>
 +
<td>TOTAL</td>
 +
<td>12</td>
 +
</tr>
 +
</table>

Revision as of 07:55, 24 October 2008

>Home | Notebook

13 September 2008 <|> 15 September 2008

Laboratory work

Team:Communication

(13/9)--->Mini prep


Transformation
Competent cells : XL10G
  • BBa_T9002[http://partsregistry.org/Part:BBa_T9002](2007)
  • Plac+RhlI+LVA
  • Plac+CinI+LVA
--->(15/9)


Team:Output


Time Responce Liquid
Digestion test

  • [http://partsregistry.org/Part:BBa_Q04510 BBa_Q04510]
  • [http://partsregistry.org/Part:BBa_I716463 BBa_I716463]
  • [http://partsregistry.org/Part:BBa_C0051 BBa_C0051]
  • [http://partsregistry.org/Part:BBa_C0070 BBa_C0070]
  • [http://partsregistry.org/Part:BBa_J06620 BBa_J06620]
  • [http://partsregistry.org/Part:BBa_J22141 BBa_J22141]
  • [http://partsregistry.org/Part:BBa_C0076 BBa_C0076]
  • BBa_I716542
  • [http://partsregistry.org/Part:BBa_I716462 BBa_I716462]
  • [http://partsregistry.org/Part:BBa_C0078 BBa_C0078]
  • [http://partsregistry.org/Part:BBa_J01101 BBa_J01101]
  • [http://partsregistry.org/Part:BBa_C0071 BBa_C0071]
  • [http://partsregistry.org/Part:BBa_I712019 BBa_I712019]
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
  • [http://partsregistry.org/Part:BBa_C0079 BBa_C0079]
  • [http://partsregistry.org/Part:BBa_C0077 BBa_C0077]
  • [http://partsregistry.org/Part:BBa_J23007 BBa_J23007]
  • [http://partsregistry.org/Part:BBa_I7100 BBa_I7100](x2)
  • [http://partsregistry.org/Part:BBa_S01416 BBa_S01416]
Sample No each
DNA(digested sample) 3
Buffer2(x10) 1
dH2O 6
EcoRⅠ 0.075
TOTAL 10

-->37℃ 1hour

Gel check

Sample No each
DNA(digestion sample) 10
Loading Dye 2
TOTAL 12
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/14_September_2008"