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Team:Chiba/Calendar-Home/26 September 2008
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(New page: >Home | Notebook 25 September 2008 <|[[Team:Chiba/Calendar-Home/27 September 2008|> 27 September 2008...)
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(New page: >Home | Notebook 25 September 2008 <|[[Team:Chiba/Calendar-Home/27 September 2008|> 27 September 2008...)
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Revision as of 09:07, 24 October 2008
25 September 2008 <|> 27 September 2008
Laboratory work
Team:Communication
Team:Output
TIME DELAY TEST
colony PCR
Ligation product(26,September)x7
Vector:BBa_R0062
| Sample No | each |
| culture | 1 |
| Fwd primer | 1.5 |
| Rev primer | 1.5 |
| Taq(DNA pol) | 0.2 |
| dNTPmix | 3 |
| thermo pol Buffer | 3 |
| dH2O | 19.8 |
| TOTAL | 30 |
->95℃ 5min ->(95℃ 1min 50℃ 30sec 72℃ 1min)x25 ->72℃ 10min
->Gel cheack
->MINI Prep
