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Team:Chiba/Calendar-Home/23 August 2008
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(New page: >Home | Notebook 22 August 2008 <|> 24 August 2008 ==Labora...) |
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===Team:Communication=== | ===Team:Communication=== | ||
:(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | :(21/8)--->'''[[Team:Chiba/protocol/DNA Purification/sigma|Mini prep]]''' | ||
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) |
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) |
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) |
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) |
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) |
| - | ::* | + | ::*[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) |
:--->'''[[Team:Chiba/protocol/digestion|Digestion test]]''' | :--->'''[[Team:Chiba/protocol/digestion|Digestion test]]''' | ||
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006) |
| - | :: | + | ::#[http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007) |
:<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | :<table width="500" border="2" cellpadding="0" cellspacing="0" bordercolor="#000000"> | ||
<tr> | <tr> | ||
| - | <td width="270">Sample No</td> | + | <td width="270">Sample No.</td> |
| - | <td> 1 | + | <td>1- 6</td><td>7</td><td>1 - 7</td><td>1 - 7</td> |
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</tr> | </tr> | ||
<tr> | <tr> | ||
| - | <td> | + | <td>DNA</td> |
| - | + | <td>3</td><td>1</td><td>3</td><td>3</td> | |
</tr> | </tr> | ||
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<tr> | <tr> | ||
<td>EcoRⅠ</td> | <td>EcoRⅠ</td> | ||
| - | + | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>PstⅠ</td> | <td>PstⅠ</td> | ||
| - | + | <td>-</td><td>-</td><td>0.1</td><td>0.1</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>Buffer EcoRⅠ</td> | <td>Buffer EcoRⅠ</td> | ||
| - | + | <td>-</td><td>-</td><td>0.9</td><td>0.8</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>BSA</td> | <td>BSA</td> | ||
| - | + | <td>-</td><td>-</td><td>1</td><td>1</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>dH2O</td> | <td>dH2O</td> | ||
| - | + | <td>-</td><td>-</td><td>5</td><td>5</td> | |
</tr> | </tr> | ||
<tr> | <tr> | ||
<td>TOTAL</td> | <td>TOTAL</td> | ||
| - | + | <td>3</td><td>1</td><td>10</td><td>10</td> | |
</tr> | </tr> | ||
</table> | </table> | ||
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::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad | ::#BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad | ||
|} | |} | ||
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===Team:Output=== | ===Team:Output=== | ||
no work | no work | ||
Revision as of 09:49, 24 October 2008
22 August 2008 <|> 24 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- (21/8)--->Mini prep
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- --->Digestion test
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2007)
- [http://partsregistry.org/Part:BBa_S03154 BBa_S03154](2006)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2007)
- [http://partsregistry.org/Part:BBa_I9026 BBa_I9026](2006)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2007)
- [http://partsregistry.org/Part:BBa_I9030 BBa_I9030](2006)
- [http://partsregistry.org/Part:BBa_J04500 BBa_J04500](2007)
Sample No. 1- 6 7 1 - 7 1 - 7 DNA 3 1 3 3 EcoRⅠ - - 0.1 0.1 PstⅠ - - 0.1 0.1 Buffer EcoRⅠ - - 0.9 0.8 BSA - - 1 1 dH2O - - 5 5 TOTAL 3 1 10 10
- --->Gel Check
Sample No 1~6 7 Sample DNA 3 1 Loading Dye 1 1 dH2O 2 4 TOTAL 6 6 - From left; Sanple No. 1~7
- BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154]
- BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154]
- BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026]
- BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026]
- BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030]
- BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030]
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]
- From left; Sanple No. 1~7
Sample No 8~21 Sample DNA 10 Loading Dye 2 TOTAL 12 - From left;
- (Single Digestion) Sanple No. 8~14
- BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
- BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
- BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
- BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
- BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
- BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
- (Double Digestion) Sample No. 15~21
- BBa_S03154(2007) [http://partsregistry.org/Part:BBa_S03154] -> OK
- BBa_S03154(2006) [http://partsregistry.org/Part:BBa_S03154] -> OK
- BBa_I9026(2007) [http://partsregistry.org/Part:BBa_I9026] -> OK
- BBa_I9026(2006) [http://partsregistry.org/Part:BBa_I9026] -> OK
- BBa_I9030(2007) [http://partsregistry.org/Part:BBa_I9030] -> OK
- BBa_I9030(2006) [http://partsregistry.org/Part:BBa_I9030] -> OK
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK?
- Digestion test
- BBa_J04500(2007:old-sample)[http://partsregistry.org/Part:BBa_J04500]
Sample No 1 2 3 Sample DNA 3 3 3 EcoRⅠ - 0.1 0.1 SpeⅠ - - 0.1 EcoRⅠ Buffer - 0.9 0.8 BSA - 1 1 dH2O - 5 5 TOTAL 3 10 10
--->Gel Check
Sample No 1 2 3 Sample DNA 3 10 10 Loading Dye 1 2 2 dH2O 2 - - TOTAL 6 12 12 - From left;
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500] -> OK
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Single Digestion -> OK
- BBa_J04500(2007) [http://partsregistry.org/Part:BBa_J04500]Double Digestion -> Bad
- From left;
Team:Output
no work
