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Team:Chiba/Calendar-Home/27 August 2008
From 2008.igem.org
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| - | ::Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate( | + | ::Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)). |
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Revision as of 10:21, 24 October 2008
26 August 2008 <|> 28 August 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
- Sample DNA :[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]
| Sample No. | 1 | 2 | 3 |
| Sample DNA | 1 | 1 | 1 |
| PstⅠ | - | - | 0.5 |
| EcoRⅠ | - | 0.5 | 0.5 |
| Buffer 3 | - | 1 | 1 |
| BSA | - | - | 1 |
| dH2O | - | 7.5 | 2 |
| TOTAL | 1 | 10 | 10 |
- --->Gel Check
|
|
- (26/8)--->Colony-PCR
- Colony PCR of 5 colonies from ligation plates (26/8-(4),(5)) and one from control plate([http://partsregistry.org/Part:BBa_F2620 BBa_F2620](2007)).
DNA Template 1 dNTP mix 5 Foward Primer 5 Reverse Primer 5 DNA polymerase 0.5 Thermopol Buffer 5 dH2O 28.5 TOTAL 50μL
- 95℃,5min -> ( 95℃,1min -> 52℃,1min -> 72℃,1min )･･･25cycles -> 72℃,10min -> 6℃
- --->Gel Check
Sample DNA 10 Loading Dye 2 TOTAL 12 - From left;
- 1~5 insert:C0170 + vector:J04500 -> OK
- 6 F2620･･Positive Control -> OK
- 7~11 insert:C0178 + vector:J04500 -> OK
- From left;
- --->(28/8)Miniprep
- competent cells : XL10G
- [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2007)
- [http://partsregistry.org/Part:BBa_C0062 BBa_C0062](2006) -> no colony on plates
- [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2007)
- [http://partsregistry.org/Part:BBa_C0179 BBa_C0179](2006)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2007)
- [http://partsregistry.org/Part:BBa_S03156 BBa_S03156](2006)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2007)
- [http://partsregistry.org/Part:BBa_S03158 BBa_S03158](2006)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S03160](2007)
- [http://partsregistry.org/Part:BBa_S03160 BBa_S0316](2006)
- --->Mini prep
- --->(29/8)Gel Check
Team:Output
no work
