Team:Warsaw/Calendar-Main/3 September 2008
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<p><ol><li>Samples were resuspended in PBS, sonicated and centrifuged.</li> | <p><ol><li>Samples were resuspended in PBS, sonicated and centrifuged.</li> | ||
<li>Lysis buffer was added separately to pellet and supernatant, then samples were boiled for 10 min. </li> | <li>Lysis buffer was added separately to pellet and supernatant, then samples were boiled for 10 min. </li> | ||
- | <li>Lysates loaded on 12% | + | <li>Lysates loaded on 12% polyacrylamide gel (amount relating to 100 μl of OD=1.0 culture).</li> |
<li>Gel stained with Coomassie Blue. Optimal induction conditions chosen.</li> | <li>Gel stained with Coomassie Blue. Optimal induction conditions chosen.</li> | ||
<li>A-alpha inoculated for overnight culture (Rosetta strain).</li></ol></p> | <li>A-alpha inoculated for overnight culture (Rosetta strain).</li></ol></p> |
Revision as of 12:37, 24 October 2008