Team:Warsaw/Calendar-Main/9 September 2008
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<h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | <h3>Tests for ampicillin resistance given by protein added to medium</h3><h4>Piotr</h4> | ||
- | <p>Inoculation of TOP10 carrying OmpA_A_alpha, OmpA_A_omega | + | <p>Inoculation of TOP10 carrying OmpA_A_alpha, OmpA_A_omega and OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.</p> |
Revision as of 14:09, 24 October 2008
Purification of proteins: A-alphaPiotrMeasurement of concentration of protein(BCA method). Tests for ampicillin resistance given by protein added to mediumPiotrInoculation of TOP10 carrying OmpA_A_alpha, OmpA_A_omega and OmpA_Z_omega to LB with inducer (0.25 mM IPTG) and kanamycin.
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