Team:Warsaw/Calendar-Main/11 September 2008

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We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!</p><br><br>
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<h3>Repetition of test for ampicillin resistance given by protein added to medium</h3><h4>Michał L., Ewa, Marcin</h4>
 
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<ol><li>We follow the <a href="https://2008.igem.org/wiki/index.php?title=Wiki/Team:Warsaw/protocols#Testing%20various%20hunter/prey%20combinations">protocol</a>.
 
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<ul><li>Inoculation of <i>E. coli</i> TOP10 carrying OmpA variants into liquid LB with kanamycin and IPTG.</li></ul></li></ol>
 
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Revision as of 15:19, 24 October 2008

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Tests for ampicillin resistance given by protein added to medium

Piotr

  1. Inoculation of OmpA_variant from previous day to LB + 0.25 mM IPTG, kanamycin and 50 μl/ml ampicylin with minimum concentration of prey (yesterday results).
  2. Mesurment of growth after 6 hours - 2x większy wzrost bakterii z białkiemo ddziałującym (Omp_A_omega 2x więcej niż Omp_omega z białkiem Z_alpha itd...)!!!
    We're fighting hard to obtain funds for the visit on iGEM2008 Jamboree!

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