Team:Chiba/Calendar-Home/8 September 2008

From 2008.igem.org

(Difference between revisions)
(→9 September, 2008)
(→Team:Output)
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[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
[[Team:Chiba/protocol/ligation/dephosphorylation|SAP]]
-
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)①
+
*[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
-
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤
+
*[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>30</td><td>30</td>
+
<td>30μl</td><td>30μl</td>
-
</tr>
+
-
<tr>
+
-
<td>TOTAL</td>
+
-
<td>30</td><td>30</td>
+
</tr>
</tr>
</table>
</table>
Line 79: Line 75:
<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>6</td>
+
<td>6μl</td>
</tr>
</tr>
</table>
</table>
-
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040](ptet)① 75ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158](RBS+RhlR)② 33.6ng/μl  
+
*insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl  
-
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156](RBS+lasR)③ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160](RBS+cinR)④ 38.4ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
-
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010](pLac)⑤ 100ng/μl
+
*vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
-
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008](RBS+rLuc)⑥ 33.6ng/μl
+
*insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
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<tr>
<tr>
<td>TOTAL</td>
<td>TOTAL</td>
-
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
+
<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td><td>20μl</td><td>10μl</td>
</tr>
</tr>
</table>
</table>

Revision as of 15:48, 24 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract


--->Zymo Clean
->15μL?


Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
Sample No. ①⑤
DNA(digested sample) 76
SAP/td> 22
SAP Buffer 33
TOTAL 30μl30μl

-->37℃ 1hour

-->65℃ 30min

-->DNA purification

-->21μl 6μl

-->Gel check

Sample No. each
DNA(digested sample) 1
Loading Dye 1
dH2O 4
TOTAL 6μl
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ①&②①&③①&④①⑤&⑥⑤
DNA(vector) 333352.1
DNA(insert) 333-3.1-
Ligase 111111
Ligase Buffer(x5) 222222
dH2O 11148.94
TOTAL 10μl10μl10μl10μl20μl10μl
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