Team:Warsaw/Calendar-Main/26 June 2008

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<h3>Change of the reporter from pZC with B-galactosidase to GFP or RFP</h3>
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<h3>Change of the reporter from <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a> with B-galactosidase to GFP or RFP</h3>
<h4>Piotr, Weronika</h4>
<h4>Piotr, Weronika</h4>
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<li>Isolation of pZC</li>
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<li>Isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a></li>
<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)
<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)

Revision as of 15:59, 24 October 2008

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Change of the reporter from pZC30 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. Isolation of pZC30
  2. Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)

Preparation of constructs with OmpA protein fusions

Michał K.

Repetition of PCRs, gel-out isolations and digests from 17 June.

Polymerase Chain Ligation (PCL)

Michał L., Ewa, Marcin

ProductTemplatesPrimersProduct length
Alpha-A fusionAlpha-linker + linker-AAlphaL+SacI + AP+NotI 1100 bp
Omega-A fusionOmega-linker + linker-AOmegaL+SacI + AP+NotI 750 bp


PCL program for fusion A with alpha and omega
TemperatureTime
94°C4:00
94°C0:3028 cycles
48°C-62°C0:45
68°C1:30
68°C10:00
4°Cinfinite

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