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Team:Warsaw/Calendar-Main/29 August 2008
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Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a>. Plating on LB with chloramphenicol and ampicillin.</p> | Single transformations of <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#rosetta">Rosetta</a> with plasmids <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pET15b%2BA-alpha>pET15b+A-alpha</a>. Plating on LB with chloramphenicol and ampicillin.</p> | ||
| - | <h3>Blue-white screening and rifampicin test in </h3> | + | <h3>Blue-white screening and rifampicin test in <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#gm">GM2163</a></h3> |
<h4>Michał L.</h4> | <h4>Michał L.</h4> | ||
| - | GM2163 competent cells carrying pZC320 were transformed with following plasmids: | + | <p>GM2163 competent cells carrying pZC320 were transformed with following plasmids: </p> |
<ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a>,</li> | <ul><li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-omega>pMPMT5-omega</a>,</li> | ||
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<li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul> | <li><a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5%2BAID%2BT7>pMPMT5-AID+T7</a>.</li></ul> | ||
| - | Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant. | + | <p>Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.</p> |
</html> | </html> | ||
Revision as of 16:27, 24 October 2008
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Cloning of protein A DNA to pET15b+Omp-alpha plasmidMichał K.
Protein purification: A-alphaPiotrSingle transformations of Rosetta with plasmids pET15b+A-alpha. Plating on LB with chloramphenicol and ampicillin. Blue-white screening and rifampicin test in GM2163Michał L.GM2163 competent cells carrying pZC320 were transformed with following plasmids: Transformants were selected on CTAXI plates (LB + Chloramphenicol + Tetracycline + IPTG + Ampicillin 30 μg/mL + X-Gal) because GM2163 are chloramphenicol resistant.
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