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Team:Warsaw/Calendar-Main/26 June 2008
From 2008.igem.org
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<li>Isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a></li> | <li>Isolation of <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC30</a></li> | ||
| - | <li>Inoculation of E.coli TOP10 carrying | + | <li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(Gfp genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator) |
</li></ol> | </li></ol> | ||
Revision as of 16:28, 24 October 2008
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Change of the reporter from pZC30 with B-galactosidase to GFP or RFPPiotr, Weronika
Preparation of constructs with OmpA protein fusionsMichał K.Repetition of PCRs, gel-out isolations and digests from 17 June. Polymerase Chain Ligation (PCL)Michał L., Ewa, Marcin
PCL program for fusion A with alpha and omega
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