Team:Warsaw/Calendar-Main/30 June 2008

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<li>Inoculation of liquid LB medium + Amp with white colonies</li>
<li>Inoculation of liquid LB medium + Amp with white colonies</li>
<li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a></li>
<li>Inoculation of 10ml LB with E.coli TOP10 carrying <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pZC320>pZC320</a></li>
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<li>Inoculation of E.coli TOP10 carrying nazwa (Gfp genetrator), nazwa (RFP generator)
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<li>Inoculation of E.coli TOP10 carrying <a href=http://partsregistry.org/wiki/index.php?title=Part:pSB1A2>pSB1A2</a> standard plasmids carrying parts: <A href=http://partsregistry.org/wiki/index.php?title=Part:BBa_E0840>BBa_E0840</a>(Gfp genetrator) and <a href=http://partsregistry.org/wiki/index.php?title=Part:BBa_J04450>BBa_J04450</a> (RFP generator)
</li></ol>
</li></ol>

Revision as of 16:32, 24 October 2008

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Change of the reporter from pZC320 with B-galactosidase to GFP or RFP

Piotr, Weronika

  1. There were blue and white colonies but no red or green in UV
  2. Inoculation of liquid LB medium + Amp with white colonies
  3. Inoculation of 10ml LB with E.coli TOP10 carrying pZC320
  4. Inoculation of E.coli TOP10 carrying pSB1A2 standard plasmids carrying parts: BBa_E0840(Gfp genetrator) and BBa_J04450 (RFP generator)

Cloning alpha-A and omega-A fusions on pKS

Michał L., Ewa, Marcin

Inoculation of liquid LB medium with 6 transformant colonies.

Preparation of constructs with OmpA protein fusions

Paweł

Inoculation of E. coli with pET15b plasmid on LB + ampicillin.

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