Team:Warsaw/Calendar-Main/16 September 2008

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<h3>'Hunter and prey' system tests</h3>
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<h3>'Hunter and prey' system tests: Competition tests</h3>
<h4>Piotr, Emilia</h4>
<h4>Piotr, Emilia</h4>
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<p>Testing of <ul><li>resistance to antibiotics achieved by interacting with a protein in medium;</li><li>minimum amount of protein in medium providing resistance to ampicillin (50 ul/ml) for OmpA with A-alpha, A-omega and Z-omega;</p></li>
 
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<p>Checking the minimal amount of protein added to the medium is sufficient for ampicillin (Ap 50) resistance for OmpA variants: A-alpha, A-omega and Z-omega.</p>
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<p>Competition tests: <br>
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Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)             
Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)             
                                                     <ul><li>A-omega, omega and Z-omega - A_alpha protein added ~100 μg</li>  
                                                     <ul><li>A-omega, omega and Z-omega - A_alpha protein added ~100 μg</li>  

Revision as of 16:59, 24 October 2008

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'Hunter and prey' system tests: Competition tests

Piotr, Emilia

Inoculation to one tube (0.25 mM IPTG; ampicillin 50 μl/ml)

  • A-omega, omega and Z-omega - A_alpha protein added ~100 μg
  • A-omega, omega and Z-omega - Z_alfa protein added ~100 μg
  • A-alpha, alpha and Z-alpha - Z_omega protein added ~100 μg

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