Team:Tokyo Tech/Construction

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== '''Construction''' ==
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<p class=MsoNormal>For checking pressure-response ability of Plac, we constructed three plasmids.Those are showed at figure 1.Plac stands for a promoter repressed by lacI protein. Ptet stands for promoter repressed by tetR protein.∆p stands for promoter less.JM109 is constitutive expression lacI protein so that Plac is always repressed by lacI protein.And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control.  </font></p>
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<p class=MsoNormal>Each parts is constructed on low-copy plasmid “pSB6” ,and inserted into E.coli strain JM109.</p></td>
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Revision as of 17:03, 24 October 2008

Home Construction Acrylic container Development of promoter Genetic toggle switch Parts Submitted to the Registry Result The Team Notebook

Construction

　

For checking pressure-response ability of Plac, we constructed three plasmids.Those are showed at figure 1.Plac stands for a promoter repressed by lacI protein. Ptet stands for promoter repressed by tetR protein.∆p stands for promoter less.JM109 is constitutive expression lacI protein so that Plac is always repressed by lacI protein.And JM109 isn’t expression tetR. Therefore, we used Ptet as a positive control.

　
　

Fig1

 　
　

Each parts is constructed on low-copy plasmid “pSB6” ,and inserted into E.coli strain JM109.

 　

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