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Team:Chiba/Calendar-Home/8 September 2008
From 2008.igem.org
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:--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]''' | :--->'''[[Team:Chiba/protocol/DNA Purification/zymocleam|Zymo Clean]]''' | ||
| - | ::: | + | :::eluted with 15μL(?) of NFW. |
| - | + | ||
| - | + | ||
===Team:Output=== | ===Team:Output=== | ||
Revision as of 02:06, 25 October 2008
7 September 2008 <|> 9 September 2008
Contents |
Laboratory work
Team:Input
no work
Team:Communication
-->(7/9)
- --->Zymo Clean
- eluted with 15μL(?) of NFW.
Team:Output
- [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
- [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
| Sample No. | ① | ⑤ | |
| DNA(digested sample) | 7 | 6 | |
| SAP/td> | 2 | 2 | |
| SAP Buffer | 3 | 3 | |
| TOTAL | 30μl | 30μl |
-->37℃ 1hour
-->65℃ 30min
-->DNA purification(①:21μl,⑤:6μl)
-->Gel check
| Sample No. | each |
| DNA(digested sample) | 1 |
| Loading Dye | 1 |
| dH2O | 4 |
| TOTAL | 6μl |
- vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
- insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
- vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
- insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
| Sample No. | ①&② | ①&③ | ①&④ | ① | ⑤&⑥ | ⑤ |
| DNA(vector) | 3 | 3 | 3 | 3 | 5 | 2.1 |
| DNA(insert) | 3 | 3 | 3 | - | 3.1 | - |
| Ligase | 1 | 1 | 1 | 1 | 1 | 1 |
| Ligase Buffer(x5) | 2 | 2 | 2 | 2 | 2 | 2 |
| dH2O | 1 | 1 | 1 | 4 | 8.9 | 4 |
| TOTAL | 10μl | 10μl | 10μl | 10μl | 20μl | 10μl |
