Team:Chiba/Calendar-Home/8 September 2008

From 2008.igem.org

(Difference between revisions)
(→Team:Communication)
(→Team:Output)
Line 27: Line 27:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)</td>
+
<td>DNA(digested sample)(μL)</td>
<td>7</td><td>6</td>
<td>7</td><td>6</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SAP/td>
+
<td>SAP(μL)</td>
<td>2</td><td>2</td>
<td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>SAP Buffer</td>
+
<td>SAP Buffer(μL)</td>
<td>3</td><td>3</td><td>
<td>3</td><td>3</td><td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>30μl</td><td>30μl</td>
+
<td>30</td><td>30</td>
</tr>
</tr>
</table>
</table>
-
-->37℃ 1hour
+
-->left at 37 degrees for 1 hour.
-
-->65℃ 30min
+
-->left at 65 degrees for 30 minutes.
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](①:21μl,⑤:6μl)
-->[[Team:Chiba/protocol/DNA_Purification/zymocleam|DNA purification]](①:21μl,⑤:6μl)
Line 59: Line 59:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(digested sample)</td>
+
<td>DNA(digested sample)(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye </td>
+
<td>Loading Dye(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>6μl</td>
+
<td>6</td>
</tr>
</tr>
</table>
</table>
Line 89: Line 89:
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(vector)</td>
+
<td>DNA(vector)(μL)</td>
<td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
<td>3</td><td>3</td><td>3</td><td>3</td><td>5</td><td>2.1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA(insert)</td>
+
<td>DNA(insert)(μL)</td>
<td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
<td>3</td><td>3</td><td>3</td><td>-</td><td>3.1</td><td>-</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase</td>
+
<td>Ligase(μL)</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
<td>1</td><td>1</td><td>1</td><td>1</td><td>1</td><td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Ligase Buffer(x5)</td>
+
<td>Ligase Buffer(x5)(μL)</td>
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
<td>2</td><td>2</td><td>2</td><td>2</td><td>2</td><td>2</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH<sub>2</sub>O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
<td>1</td><td>1</td><td>1</td><td>4</td><td>8.9</td><td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
-
<td>10μl</td><td>10μl</td><td>10μl</td><td>10μl</td><td>20μl</td><td>10μl</td>
+
<td>10</td><td>10</td><td>10</td><td>10</td><td>20</td><td>10</td>
</tr>
</tr>
</table>
</table>

Revision as of 02:10, 25 October 2008

>Home | Notebook

7 September 2008 <|> 9 September 2008

Contents

Laboratory work

Team:Input

no work

Team:Communication

-->(7/9)

Gel Extract


--->Zymo Clean
eluted with 15μL(?) of NFW.

Team:Output

SAP

  • [http://partsregistry.org/Part:BBa_R0040 BBa_R0040]①
  • [http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤
Sample No. ①⑤
DNA(digested sample)(μL) 76
SAP(μL) 22
SAP Buffer(μL) 33
TOTAL(μL) 3030

-->left at 37 degrees for 1 hour.

-->left at 65 degrees for 30 minutes.

-->DNA purification(①:21μl,⑤:6μl)


-->Gel check

Sample No. each
DNA(digested sample)(μL) 1
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
  • vector:[http://partsregistry.org/Part:BBa_R0040 BBa_R0040]① 75ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03158 BBa_S03158]② 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03156 BBa_S03156]③ 33.6ng/μl
  • insert:[http://partsregistry.org/Part:BBa_S03160 Ba_S03160]④ 38.4ng/μl
  • vector:[http://partsregistry.org/Part:BBa_R0010 BBa_R0010]⑤ 100ng/μl
  • insert:[http://partsregistry.org/Part:BBa_J52008 BBa_J52008]⑥ 33.6ng/μl
Sample No. ①&②①&③①&④①⑤&⑥⑤
DNA(vector)(μL) 333352.1
DNA(insert)(μL) 333-3.1-
Ligase(μL) 111111
Ligase Buffer(x5)(μL) 222222
dH2O(μL) 11148.94
TOTAL(μL) 101010102010
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/8_September_2008"