Team:Chiba/Calendar-Home/18 September 2008

From 2008.igem.org

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(→Team:Output)
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[[Team:Chiba/protocol/gelcheck|Gel check]]
[[Team:Chiba/protocol/gelcheck|Gel check]]
*Ligation product(Venus YFP)
*Ligation product(Venus YFP)
-
*BBa_J37033
+
*[http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
-
*BBa_J37020
+
*[http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
-
*BBa_J37032
+
*[http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
 +
 
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<table width="250" border="4" cellpadding="0" cellspacing="0" bordercolor="#000000">
<tr>
<tr>
-
<td width="257">Sample No</td>
+
<td width="257">Sample No.</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>DNA</td>
+
<td>DNA(μL)</td>
<td>each</td>
<td>each</td>
</tr>
</tr>
<tr>
<tr>
-
<td>Loading Dye</td>
+
<td>Loading Dye(μL)</td>
<td>1</td>
<td>1</td>
</tr>
</tr>
<tr>
<tr>
-
<td>dH2O</td>
+
<td>dH<sub>2</sub>O(μL)</td>
<td>4</td>
<td>4</td>
</tr>
</tr>
<tr>
<tr>
-
<td>TOTAL</td>
+
<td>TOTAL(μL)</td>
<td>6</td>
<td>6</td>
</tr>
</tr>
</table>
</table>

Revision as of 03:39, 25 October 2008

>Home | Notebook

17 September 2008 <|> 19 September 2008

Laboratory work

Team:Communication

  1. strain
    Senders:XL10GOLD
    Receiver:JW1908
  2. temparature
    37℃ and 30℃


Team:Output

SAP 1μL

->left for:

  • 1 hour at 37 degrees
  • 30 minutes at 65 degrees.

Zymoclean

->eluted with 25μL of NFW.

Ligation

  • vector:[http://partsregistry.org/Part:BBa_I7100 BBa_I7100]
  • insert:[http://partsregistry.org/Part:BBa_S03119 BBa_S03119](ptet+LuxR)
  • Ligation product(ptet+LasR,ptet+RhlR,ptet+cinR)
Sample No. Vector&insertVector
DNA(vector)(μL) 3.53.5
DNA(insert)(μL) 7.5-
Ligase(μL) 11
Ligase Buffer(μL) 44
dH2O(μL) 44
TOTAL(μL) 2020

transformation

  • [http://partsregistry.org/Part:BBa_J54103 BBa_J54103](RBS+GFP)
  • [http://partsregistry.org/Part:BBa_P2006 BBa_P2006]

Gel check

  • Ligation product(Venus YFP)
  • [http://partsregistry.org/Part:BBa_J37033 BBa_J37033]
  • [http://partsregistry.org/Part:BBa_J37020 BBa_J37020]
  • [http://partsregistry.org/Part:BBa_J37032 BBa_J37032]
Sample No. each
DNA(μL) each
Loading Dye(μL) 1
dH2O(μL) 4
TOTAL(μL) 6
Retrieved from "http://2008.igem.org/Team:Chiba/Calendar-Home/18_September_2008"