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| - | <html><link rel="stylesheet" href="https://2008.igem.org/wiki/index.php?title=User:Hiroki/style.css&action=raw&ctype=text/css" type="text/css" /></html>
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| - | [[Image:Chiba-U.gif]]
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| - | {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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| - | !align="center"|[[Team:Chiba|Home]]
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| - | !align="center"|[[Team:Chiba/Team|The Team]]
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| - | !align="center"|[[Team:Chiba/Project|The Project]]
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| - | !align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:Chiba/Notebook|Notebook]]
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| - | |}
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| - | {| style="border:0;width:100%;font-family:'Trebuchet MS'" cellpadding="20px" cellspacing="0"
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| - | | align="center" |
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| - | <span style="font-size:120%;font-weight:bold;">[[Chiba/Project_Design|Project Design]] ( [[Chiba/Sender_experiments|Sender 実験]] | [[Chiba/Receiver_experiments|レシーバー実験]] ) [[Chiba/Making time_delay|Making time delay]] | [[Chiba/Goal|Our Goal]]</span><br>
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| - | |}
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| - | ===Quorum-Sensing Cross-talk===
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| - | *'''Senders'''
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| - | **[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
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| - | [[Image:Tet-lasi chiba.gif]]
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| - | **[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
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| - | [[Image:Tet-rhli chiba.gif]]
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| - | **[http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
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| - | [[Image:Tet-luxi chiba.gif]]
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| - | *'''Receiver'''
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| - | **[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
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| - | [[Image:Reporter 9002 chiba.gif]]
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| - | ===Method===
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| - | To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
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| - | #Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
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| - | #Inoculated them independently in liquid media. Incubated at 37℃ 12h
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| - | #Mixed them.
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| - | #Incubated at 37℃ or 30℃.
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| - | #Measured intensity of green fluorescence at regular time intervals.
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| - | ===Result===
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| - | {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"
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| - | !align="center"|[[Team:Chiba|Home]]
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| - | !align="center"|[[Team:Chiba/Team|The Team]]
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| - | !align="center"|[[Team:Chiba/Project|The Project]]
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| - | !align="center"|[[Team:Chiba/Parts|Parts Submitted to the Registry]]
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| - | !align="center"|[[Team:Chiba/Notebook|Notebook]]
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| - | |}
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