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Team:Chiba/Sender experiments
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| - | <span style="font-size:120%;font-weight:bold;">[[Chiba/ | + | <span style="font-size:120%;font-weight:bold;">[[Team:Chiba/Project|Project Design]] ( [[Team:Chiba/Sender_experiments|Sender 実験]] | [[Team:Chiba/Receiver_experiments|レシーバー実験]] ) [[Team:Chiba/Making time_delay|Making time delay]] | [[Team:Chiba/Goal|Our Goal]]</span><br> |
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===Quorum-Sensing Cross-talk=== | ===Quorum-Sensing Cross-talk=== | ||
Revision as of 05:00, 25 October 2008
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Project Design ( Sender 実験 | レシーバー実験 ) Making time delay | Our Goal |
Quorum-Sensing Cross-talk
- Senders
- [http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
- [http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
- [http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
- Receiver
- [http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
Method
To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.
- Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
- Inoculated them independently in liquid media. Incubated at 37℃ 12h
- Mixed them.
- Incubated at 37℃ or 30℃.
- Measured intensity of green fluorescence at regular time intervals.
Result
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