Team:Chiba/Sender experiments

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Revision as of 07:03, 25 October 2008

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Fig.5 Senders crosstalk

[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]

To characterize quorum sensing crosstalk, constitutive AHL senders were mixed with constitutive receivers and measure fluorescence intensity.

Transformed Senders into E.coli strains(JW1908/XL10GOLD) and Receiver into E.coli strain(JW1908).
Inoculated them independently in liquid media. Incubated at 37°C 12h
Mixed them.
Incubated at 37°C or 30°C.
Measured intensity of green fluorescence at regular time intervals.

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@@ Line 16: / Line 16: @@
 ==Quorum-Sensing Cross-talk==
+===Purpose===
 [[Image:Sender crosstalk chiba.gif|frame|right|'''Fig.5''' Senders crosstalk]]
-*'''Senders'''
+===='''Senders'''====
-**[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
+*[http://partsregistry.org/Part:BBa_K084007 plac+rbs+LasI]
 [[Image:Tet-lasi chiba.gif]]
-**[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
+*[http://partsregistry.org/Part:BBa_K084008 plac+rbs+RhlI]
 [[Image:Tet-rhli chiba.gif]]
-**[http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
+*[http://partsregistry.org/Part:BBa_K084012 plac+rbs+LuxI]
 [[Image:Tet-luxi chiba.gif]]
-*'''Receiver'''
+===='''Receiver'''====
-**[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
+*[http://partsregistry.org/Part:BBa_T9002 BBa_T9002 (Express GFP in response to AHL)]
 [[Image:Reporter 9002 chiba.gif]]
@@ Line 39: / Line 41: @@
 ===Result===
 {| style="color:white;background-color:Maroon" cellpadding="3" cellspacing="3" border="1" bordercolor="white" width="100%" align="center"