Team:Warsaw/Calendar-Main/22 May 2008

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Revision as of 09:26, 25 October 2008


	Attribution analyzer / Interactive list of topics

Rifampicin Test

Piotr

Overnight inoculation and induction:
Top10
and Top10 carrying:
pMPM-T5-AID + 0.01% L-Ara
pTrc99a-AID + 0.5 mM IPTG
pMPM-T5-AID
pTrc99a-AID

Preparation of pMPMT5-AID+T7 and pMPMT5+AID-T7

Piotr

Design of primers for sequencing something we want from pMPM-T5.
Primers:

Michał K.

Isolation of hypothetical pMPM-T5 with transcriptional fusion.
Isolation of hypothetical pMPM-T5 with translational fusion
Control digest of pMPM-T5 with transcriptional fusion with EcoRI (EcoRI buffer).
Control digest of pMPM-T5 with translational fusion with BamHI (BamHI buffer).
Gel electrophoresis (no proper colonies found). Fig. 1.

Fig. 1. 1-DNA ladder; 2 and 3-hypothetical pMPM-T5 with transcriptional fusion digested with EcoRI (2 clones); 4-pMPM-T5-AID digested with EcoRI; 5-hypothetical pMPM with translational fusion digested with BamHI; 6-pMPM-T5-AID digested with BamHI.

@@ Line 10: / Line 10: @@
 <p>Overnight inoculation and induction:<br>
 <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a><br>
+and <a href="https://2008.igem.org/Wiki/Team:Warsaw/igem_notebook.htm#top10">Top10</a> carrying:<br>
 <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pMPM-T5-AID>pMPM-T5-AID</a> + 0.01% L-Ara<br>
 <a href=https://2008.igem.org/Wiki/Team:Warsaw/vectors/pTrc99A-AID>pTrc99a-AID</a> + 0.5 mM IPTG<br>